Equipment

Electron microscopy

  • SEM Magellan 400/L (ThermoFisher)
    • cryo-stage with adapter VCT100 (Leica microsystems) with optional extension to cooling down to -30K using liquid Helium
    • EDX detector Octane (EDAX)
    • CL detector MonoCL4+ (Gatan)
    • CL detector SPARC (Delmic)
  • DualBeam FIB-SEM Helios (ThermoFisher)
    • cryo-stage with adapter VCT100 (Leica microsystems) with optional extension to cooling down to -30K using liquid Helium
    • CL detector SPARC (Delmic)

Sample preparation

  • High-pressure-freezer EM ICE (Leica microsystems)
    • Device enabling cutting-edge method of fixation of biological samples using freezing under high pressure, so-called high-pressure freezing. This type of freezing forms vitrified ice, which, unlike crystalline ice, does not damage the cells and even enables fixation of bigger samples.
  • Freeze-substitution unit EM AFS2 (Leica microsystems)
    • Freeze substitution unit enabling transition of biological samples from cryo to room temperature electron microscopy.
  • Cryo-vacuum chamber ACE600 (Leica microsystems)
    • Cryo sample preparation chamber enabling freeze fracturing and etching together with coating by evaporated carbon or metal sputtering (Pt, Au, Au/Pd, Cr, W, Ir).
  • Cryo-vacuum chamber BAF060 (Leica microsystems)
    • Cryo sample preparation chamber enabling coating the surface by evaporated C or Pt/C under high vacuum with possibilities of angle tilt and rotation during the evaporation. This method of metal coating offers one of the finest surface treatments for highlighting the ultrastructure of samples.
  • Ultramicrotome UC ENUITY (Leica microsystems)
  • Automatic Plunge Freezing EM GP2 (Leica microsystems)

Raman microscopy

  • inVia (Renishaw)
    • instrument for analysis of microbial cells and their response to external stimuli based on Raman-fingerprint
  • Raman tweezers
    • instrument enabling trapping, sorting and analysis of single cells based on Ramman signal
  • Microfluidic devices (different options, developed in-house)

Data analysis

  • 3D data analysis using the Amira software, labVIEW, MATLAB
  • In-house developed Raman spectrum database „RamanBase“

Virtual 3D tour to laboratories

Price list and General Terms and Conditions